| Autor: |
Vanessa Susini, Laura Caponi, Veronica Lucia Rossi, Antonio Sanesi, Nadia Romiti, Aldo Paolicchi, Maria Franzini |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Data in Brief, Vol 35, Iss , Pp 106778- (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2352-3409 |
| DOI: |
10.1016/j.dib.2021.106778 |
| Popis: |
The data here presented are related to the research article entitled “Sensitivity and reproducibility enhancement in enzyme immunosorbent assays based on half fragment antibodies” [1] aimed to compare the performance in ELISA of whole antibodies and their corresponding monovalent half-fragments obtained by reduction. Half-fragment antibodies represent an interesting method to orient antibodies in high-sensitive immunoassays taking advantage of the free sulfhydryl groups of the hinge region [2–4] that allow their oriented binding on maleimide functionalized microplates. Data here presented describe the contribution of both chemical reduction and orientation on the antigen binding capacity of whole and half-fragments antibodies. For this purpose, monoclonal anti-horseradish peroxidase (anti-HRP) or monoclonal anti-fPSA antibodies, and their respective half-fragments, were coated on polystyrene or maleimide functionalized microplates. The antigen binding capability was analyzed by in-house enzyme linked immunosorbent assays. These data would be used for further studies on the development of oriented immunoassays based on half fragment antibodies. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
