L-carnitine supplemented extenders improve post-thawing quality of honey bee drone (apis mellifera) spermatozoa

Autor: Selim ALCAY, Selvinar CAKMAK, Ibrahim CAKMAK, Ahmet AKTAR, Melih YILMAZ, Burcu USTUNER, Mustafa AKKASOGLU, Seyma TASKIRAN, Elif AYAZ, Hakan SAGIRKAYA, Zekariya NUR
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Kafkas Universitesi Veteriner Fakültesi Dergisi, Vol 27, Iss 4, Pp 489-493 (2021)
Druh dokumentu: article
ISSN: 1309-2251
DOI: 10.9775/kvfd.2021.25756
Popis: The study aimed to evaluate the eff ects of L-carnitine on honey bee (Apis mellifera) drone spermatozoon at post-thaw. Semen samples obtained from sexually mature drones were pooled. Then, pooled semen was diluted with diff erent concentrations of L-carnitine (2.5 mM/ 5 mM/ 10 mM) and without L-carnitine (control) supplemented extenders. Motility, plasma membrane functional integrity, acrosomal integrity and mitochondrial function were negatively aff ected by the cryopreservation process but DNA integrity was not aff ected. Malondialdehyde (MDA) concentration was used for analyzing the membrane lipid peroxidation status and a better result was obtained in the LC5 group compared with the control group at post-thaw. L-carnitine groups had a positive eff ect on sperm motility, plasma membrane integrity, and mitochondrial function compared to the control group (P
Databáze: Directory of Open Access Journals