| Autor: |
Maryam Ghahramani, Reza Yousefi, Kazem Khoshaman, Sogand Sasan Moghadam, Boris Kurganov |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Data in Brief, Vol 30, Iss , Pp 105492- (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2352-3409 |
| DOI: |
10.1016/j.dib.2020.105492 |
| Popis: |
The interaction of αA- and αB-crystallins with Cu2+ ion modulates their structure and chaperone-like activity which is important for lens transparency. Theoretical analysis of the dependences of fluorescence intensity of native αA- and αB-crystallins and αA- and αB-crystallins modified by peroxynitrite on concentration of Cu2+ ions has been carried out. It has been shown that one subunit of native αA-crystallin contains two equivalent Cu2+-binding sites. The microscopic dissociation constant for Cu2+–αA-crystallin complex (Kdiss) was found to be equal to 9.7 µM. For peroxynitrite modified αA-crystallin the Kdiss value is equal to 17 µM. One subunit of native αB-crystallin contains two non-equivalent Cu2+-binding sites. The corresponding microscopic dissociation constants for Cu2+–αB-crystallin complexes (K1 and K2) were found to be equal to 0.94 and 36 µM. For peroxynitrite modified αB-crystallin the K1 and K2 values are equal to 4.3 and 70 µM, respectively. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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