| Autor: |
Svenja Memmert, Marjan Nokhbehsaim, Anna Damanaki, Andressa V. B. Nogueira, Alexandra K. Papadopoulou, Christina Piperi, Efthimia K. Basdra, Birgit Rath-Deschner, Werner Götz, Joni A. Cirelli, Andreas Jäger, James Deschner |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
BMC Oral Health, Vol 18, Iss 1, Pp 1-7 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
1472-6831 |
| DOI: |
10.1186/s12903-018-0518-2 |
| Popis: |
Abstract Background Cathepsin S is a cysteine protease, which is expressed in human periodontal ligament (PDL) cells under inflammatory and infectious conditions. This in vitro study was established to investigate the effect of cathepsin S on PDL cell wound closure. Methods An in vitro wound healing assay was used to monitor wound closure in wounded PDL cell monolayers for 72 h in the presence and absence of cathepsin S. In addition, the effects of cathepsin S on specific markers for apoptosis and proliferation were studied at transcriptional level. Changes in the proliferation rate due to cathepsin S stimulation were analyzed by an XTT assay, and the actions of cathepsin S on cell migration were investigated via live cell tracking. Additionally, PDL cell monolayers were treated with a toll-like receptor 2 agonist in the presence and absence of a cathepsin inhibitor to examine if periodontal bacteria can alter wound closure via cathepsins. Results Cathepsin S enhanced significantly the in vitro wound healing rate by inducing proliferation and by increasing the speed of cell migration, but had no effect on apoptosis. Moreover, the toll-like receptor 2 agonist enhanced significantly the wound closure and this stimulatory effect was dependent on cathepsins. Conclusions Our findings provide original evidence that cathepsin S stimulates PDL cell proliferation and migration and, thereby, wound closure, suggesting that this cysteine protease might play a critical role in periodontal remodeling and healing. In addition, cathepsins might be exploited by periodontal bacteria to regulate critical PDL cell functions. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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