Membrane Lipid Composition Influences the Hydration of Proton Half-Channels in FoF1-ATP Synthase

Autor: Leonid A. Ivontsin, Elena V. Mashkovtseva, Yaroslav R. Nartsissov
Jazyk: angličtina
Rok vydání: 2023
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Zdroj: Life, Vol 13, Iss 9, p 1816 (2023)
Druh dokumentu: article
ISSN: 13091816
2075-1729
DOI: 10.3390/life13091816
Popis: The membrane lipid composition plays an important role in the regulation of membrane protein activity. To probe its influence on proton half-channels’ structure in FoF1-ATP synthase, we performed molecular dynamics simulations with the bacterial protein complex (PDB ID: 6VWK) embedded in three types of membranes: a model POPC, a lipid bilayer containing 25% (in vivo), and 75% (bacterial stress) of cardiolipin (CL). The structure proved to be stable regardless of the lipid composition. The presence of CL increased the hydration of half-channels. The merging of two water cavities at the inlet half-channel entrance and a long continuous chain of water molecules directly to cAsp61 from the periplasm were observed. Minor conformational changes in half-channels with the addition of CL caused extremely rare direct transitions between aGlu219-aAsp119, aGlu219-aHis245, and aGln252-cAsp61. Deeper penetration of water molecules (W1–W3) also increased the proton transport continuity. Stable spatial positions of significant amino acid (AA) residue aAsn214 were found under all simulation conditions indicate a prevailing influence of AA-AA or AA-W interactions on the side-chain dynamics. These results allowed us to put forward a model of the proton movement in ATP synthases under conditions close to in vivo and to evaluate the importance of membrane composition in simulations.
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