Nucleo-cytoplasmic shuttling of 14-3-3 epsilon carrying hnRNP C promotes autophagy
| Autor: | Manlan Guo, Minyi He, Yi Zhang, Weiwen Liu, Min Qi, Zhifeng Liu, Guozhong Yi, Shengze Deng, Yaomin Li, Xuegang Sun, Liang Zhao, Tengxiang Chen, Yawei Liu |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Cancer Biology & Therapy, Vol 24, Iss 1 (2023) |
| Druh dokumentu: | article |
| ISSN: | 1538-4047 1555-8576 15384047 |
| DOI: | 10.1080/15384047.2023.2246203 |
| Popis: | Translocation of 14-3-3 protein epsilon (14-3-3ε) was found to be involved in Triptolide (Tp)-induced inhibition of colorectal cancer (CRC) cell proliferation. However, the form of cell death induced by 14-3-3ε translocation and mechanisms underlying this effect remain unclear. This study employed label-free LC-MS/MS to identify 14-3-3ε-associated proteins in CRC cells treated with or without Tp. Our results confirmed that heterogeneous nuclear ribonucleoproteins C1/C2 (hnRNP C) were exported out of the nucleus by 14-3-3ε and degraded by ubiquitination. The nucleo-cytoplasmic shuttling of 14-3-3ε carrying hnRNP C mediated Tp-induced proliferation inhibition, cell cycle arrest and autophagic processes. These findings have broad implications for our understanding of 14-3-3ε function, provide an explanation for the mechanism of nucleo-cytoplasmic shuttling of hnRNP C and provide new insights into the complex regulation of autophagy. |
| Databáze: | Directory of Open Access Journals |
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