| Autor: |
Xuejiao Zhou, Yu Yang, Zhenzhen Tai, Haiqing Zhang, Juan Yang, Zhong Luo, Zucai Xu |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Epilepsia Open, Vol 9, Iss 4, Pp 1252-1264 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2470-9239 |
| DOI: |
10.1002/epi4.12945 |
| Popis: |
Abstract Objective The objective of this study is to determine whether inhibition of mitophagy affects seizures through Clathrin‐mediated endocytosis (CME). Methods Pentylenetetrazol (PTZ) was intraperitoneally injected daily to establish a chronic PTZ‐kindled seizure. The Western blot (WB) was used to compare the differences in Parkin protein expression between the epilepsy group and the control group. Immunofluorescence was used to detect the expression of MitoTracker and LysoTracker. Transferrin‐Alexa488 (Tf‐A488) was injected into the hippocampus of mice. We evaluated the effect of 3‐methyladenine (3‐MA) on epilepsy behavior through observation in PTZ‐kindled models. Results The methylated derivative of adenine, known as 3‐MA, has been extensively utilized in the field of autophagy research. The transferrin protein is internalized from the extracellular environment into the intracellular space via the CME pathway. Tf‐A488 uses a fluorescent marker to track CME. Western blot showed that the expression of Parkin was significantly increased in the PTZ‐kindled model (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
Plný text