| Autor: |
Pushkal Sinduvadi Ramesh, Rahul Vyas, Ruthvik Satishchandra, Arun Brunda, K B Suma, Suma M Nataraj, Avinash Shetty, Veena Shetty, Devanand Devegowda |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Journal of Clinical Virology Plus, Vol 2, Iss 4, Pp 100105- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2667-0380 |
| DOI: |
10.1016/j.jcvp.2022.100105 |
| Popis: |
Background: Human papillomavirus (HPV) is known to be associated with both cervical and oropharyngeal cancers, yet little are known about the co-occurrence of similar HPV subtypes in these subsites. With the changing sexual behaviour it is necessary to evaluate the risk of a woman with abnormal cervix developing cancer of the oropharynx. A study evaluating HPV infection in both oral mucosa and the cervix is therefore needed to understand the co-occurrence of HPV infection in 2 different sites Methods: Oral mucosa and cervical wash specimens were collected from a total of 100 women aged 20-60 years who visited for routine Pap test. DNA was extracted and then subjected to an in house real-time PCR and conventional nested PCR for the detection high risk HPV 16/18 subtypes. A limit of detection was established using serially diluted HPV positive DNA from cell lines and the sensitivity and specificity of both the assays were evaluated Results: The prevalence of HPV-16/18 in the cervical samples (19.7% vs 6.2%) and the oral mucosa (16.6% vs nil) were higher as detected by our in house real-time PCR in comparison to the conventional nested PCR. Oral HPV infection was found in 25% of the women with SCC of the cervix Conclusion: Due to the changing sexual behaviour there is a possibility that women with HPV-related cervical lesions can easily transfer the virus to the partner or even self-inoculate which might lead to the development of lesions in the head & neck turning into cancer. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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