Neuroblastoma-secreted exosomes carrying miR‐375 promote osteogenic differentiation of bone-marrow mesenchymal stromal cells

Autor: Marta Colletti, Luigi Tomao, Angela Galardi, Alessandro Paolini, Virginia Di Paolo, Cristiano De Stefanis, Paolo Mascio, Francesca Nazio, Stefania Petrini, Aurora Castellano, Ida Russo, Roberta Caruso, Simone Piga, Rita De Vito, Luisa Pascucci, Hector Peinado, Andrea Masotti, Franco Locatelli, Angela Di Giannatale
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Journal of Extracellular Vesicles, Vol 9, Iss 1 (2020)
Druh dokumentu: article
ISSN: 2001-3078
20013078
DOI: 10.1080/20013078.2020.1774144
Popis: Bone marrow (BM) is the major target organ for neuroblastoma (NB) metastasis and its involvement is associated with poor outcome. Yet, the mechanism by which NB cells invade BM is largely unknown. Tumour microenvironment represents a key element in tumour progression and mesenchymal stromal cells (MSCs) have been recognized as a fundamental part of the associated tumour stroma. Here, we show that BM-MSCs isolated from NB patients with BM involvement exhibit a greater osteogenic potential than MSCs from non-infiltrated BM. We show that BM metastasis-derived NB-cell lines secrete higher levels of exosomal miR-375, which promotes osteogenic differentiation in MSCs. Of note, clinical data demonstrate that high level of miR-375 correlates with BM metastasis in NB patients. Our findings suggest, indeed, a potential role for exosomal miR-375 in determining a favourable microenvironment in BM to promote metastatic progression. MiR-375 may, thus, represent a novel biomarker and a potential target for NB patients with BM involvement.
Databáze: Directory of Open Access Journals
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