| Autor: |
Suryono Suryono, Prayudha Benni Setiawan, Nur Rahman Ahmad Seno Aji, Christia Aye Waindy Vega, Budi Rodestawati, Endang Lukitaningsih, Friska Ani Rahman, Sherlina Devina, Sholiha Sarah Tsabita, Selcaria Istna Datau, Ananto Ali Alhasyimi |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Pharmacia, Vol 71, Iss , Pp 1-7 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2603-557X |
| DOI: |
10.3897/pharmacia.71.e118072 |
| Popis: |
Aim of the study: To investigate the potential of 10% propolis-based toothpaste on inhibiting biofilm-forming bacteria growth in vitro. Material and method: Organoleptic properties are evaluated, considering color, odor, and taste. Antibacterial tests use a disc diffusion method against Streptococcus mutans, Staphylococcus aureus and Porphyromonas gingivalis bacteria, while cytotoxicity is assessed using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay on fibroblast cells. Statistical analysis involves mean ± standard deviation. The data were then tested using a one-way analysis of variance and Kruskal-Wallis, followed by post-hoc test (p < 0.05). Results: The organoleptic evaluation of 10% propolis toothpaste reveals a visually clear appearance, consistent orange flavor, and aroma lasting 30 days. Based on the antibacterial results, a 10% level of propolis toothpaste sample inhibited the growth of Streptococcus mutans, Staphylococcus aureus and Porphyromonas gingivalis bacteria. The post-hoc test showed that toothpaste demonstrated significant inhibition on S. mutans and S. aureus compared to the negative control (p < 0.05). The toothpaste showed a larger inhibitory zone towards P. gingivalis compared to the adverse control; however, no significant differences were observed (p > 0.05). Cytotoxicity assessment on fibroblast cells shows a high percentage (85.31%) of viable cells. The findings highlight the 10% of propolis toothpaste’s potential and non-toxic as oral care product. Conclusions: 10% propolis toothpaste inhibits S. mutans, S. aureus, P. gingivalis growth, and not toxic on fibroblast. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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