Comparison of methods for evaluating genetic differentiation of populations by microsatellite markers
| Autor: | V. M. Kuznetsov |
|---|---|
| Jazyk: | ruština |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Аграрная наука Евро-Северо-Востока, Vol 21, Iss 2, Pp 169-182 (2020) |
| Druh dokumentu: | article |
| ISSN: | 2072-9081 2500-1396 |
| DOI: | 10.30766/2072-9081.2020.21.2.169-182 |
| Popis: | Comparative studies of eight methods for evaluation of genetic differences by microsatellite (STR) markers (11 loci) DNA were carried out using the example of seven breed samples (N = 84) of dairy cattle. The range of indicators of genetic diversity of samples was as follows: the number of alleles per locus 3.5-6.2, the number of effective alleles per locus – 2.4-4.3, the Shannon index – 0.95-1.56, the observed and expected heterozygosity – 0.56-0.97 and 0.53-0.75. Evaluation summary of genetic differentiation by group A methods (FST, GST, and GST(NEI)) were 13.4, 10.3, and 11.8% (pperm < 0.001); the differences between the estimates were statistically insignificant. Estimates by group B methods (G'ST(HED), G''ST(HED), DEST) were 36.4, 37.5 and 29.2% (pperm < 0.001); differences between the estimates were also statistically insignificant. Estimates obtained by group B methods were statistically significantly higher than those obtained by group A methods by almost 3 times. Methods of groups A, B, and C (GDN and uGDN) were used to calculate paired genetic distances in the samples. Despite significant dif-ferences in estimates, the Mantel test showed a high degree of correspondence of the genetic distance matrices (RM≥0.97; pperm < 0.001), which was manifested in the projections of genetic relations of breed samples on plane 1 and 2 of the principal coordinates. The first two principal coordinates explained 97-99% of STR variation in the genetic distance matrices. It can be assumed that when assessing the actual differentiation of populations by STR-markers, the methods of group B should be used and among them the statistics of DEST as independent of the level of mean within-subpopulation heterozygosity. In the study of spatial ordination of gene pools of populations, in all probability, it is reasonably to use any method. |
| Databáze: | Directory of Open Access Journals |
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