Sex determination of cattle meat by polymerase chain reaction amplification of the amelogenin (AMELX/AMELY) gene
| Autor: | P. Gokulakrishnan, R. R. Kumar, B. D. Sharma, S. K. Mendiratta, D. Sharma, O. P. Malav |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Veterinary World, Vol 5, Iss 9.000, Pp 526-529 (2012) |
| Druh dokumentu: | article |
| ISSN: | 0972-8988 2231-0916 |
| DOI: | 10.5455/vetworld.2012.526-529 |
| Popis: | Aim: The objective of this study was to develop a simplified, efficient, and accurate protocol for sexing of cattle meat based on the amelogenin (AMELX/AMELY) gene using PCR technique which is superior to earlier work in terms of band patterns. Materials and Methods: Based on the amelogenin gene located on the conservation region of X and Y chromosomes, a pair of primers was designed and the system of PCR was established to amplify a 241-bp fragment from the X chromosome in female cattle, and a 241-bp fragment from X chromosome and 178-bp fragment from the Y chromosome in male cattle, respectively. The accuracy and specificity of the primers was assessed using DNA template extracted from cattle meat samples of known sex. The protocol was subjected to a blind test showed 100% concordance, proving its accuracy and reliability. Results: PCR products of cattle meat samples after electrophoresis showed two bands (241, 178-bp) for tissue from male while female tissue resulted in only one (241-bp) band. Conclusions: Our findings show that the PCR assay based on the amelogenin gene is reliable for sex determination in cattle meat. [Vet World 2012; 5(9.000): 526-529] |
| Databáze: | Directory of Open Access Journals |
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