| Autor: |
Dorothea Reimer, Michael Meyer-Hermann, Asylkhan Rakhymzhan, Tobit Steinmetz, Philipp Tripal, Jana Thomas, Martin Boettcher, Dimitrios Mougiakakos, Sebastian R. Schulz, Sophia Urbanczyk, Anja E. Hauser, Raluca A. Niesner, Dirk Mielenz |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Cell Reports, Vol 32, Iss 6, Pp 108030- (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2211-1247 |
| DOI: |
10.1016/j.celrep.2020.108030 |
| Popis: |
Summary: Plasma cells secreting affinity-matured antibodies develop in germinal centers (GCs), where B cells migrate persistently and directionally over defined periods of time. How modes of GC B cell migration influence plasma cell development remained unclear. Through genetic deletion of the F-actin bundling protein Swiprosin-1/EF-hand domain family member 2 (EFhd2) and by two-photon microscopy, we show that EFhd2 restrains B cell speed in GCs and hapten-specific plasma cell output. Modeling the GC reaction reveals that increasing GC B cell speed promotes plasma cell generation. Lack of EFhd2 also reduces contacts of GC B cells with follicular dendritic cells in vivo. Computational modeling uncovers that both GC output and antibody affinity depend quantitatively on contacts of GC B cells with follicular dendritic cells when B cells migrate more persistently. Collectively, our data explain how GC B cells integrate speed and persistence of cell migration with B cell receptor affinity. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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