| Autor: |
Xiaoyan Li, Yiyan Lu, Xiaojun Ou, Sijing Zeng, Ying Wang, Xiaoxiao Qi, Lijun Zhu, Zhongqiu Liu |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
PeerJ, Vol 8, p e10182 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2167-8359 |
| DOI: |
10.7717/peerj.10182 |
| Popis: |
Background This study aimed to systematically profile the alterations and sex- and age-related differences in the drug metabolizing enzymes (DMEs) in a KRAS-mutant mouse model of lung cancer (KRAS mice). Methodology In this study, the LC-MS/MS approach and a probe substrate method were used to detect the alterations in 21 isoforms of DMEs, as well as the enzymatic activities of five isoforms, respectively. Western blotting was applied to study the protein expression of four related receptors. Results The proteins contents of CYP2C29 and CYP3A11, were significantly downregulated in the livers of male KRAS mice at 26 weeks (3.7- and 4.4-fold, respectively, p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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