| Autor: |
Marzieh Anjomshoa, Bagher Amirheidari, Jan Janczak, Mehdi Sahihi, Yasaman Abolhassani, Alireza Farsinejad, Hamid Forootanfar |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Scientific Reports, Vol 14, Iss 1, Pp 1-19 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2045-2322 |
| DOI: |
10.1038/s41598-024-79644-0 |
| Popis: |
Abstract Breast cancer (BC) is one of the most life-threatening diseases of women’s health worldwide. This work was conducted to assess the anti-BC potency of a new Zn(II)-based complex. The Zn(II) complex coordinated to dimethoxy-substituted bipyridine was synthesized and its molecular structure was elucidated as [Zn(2Meobpy)3](clo4)2 ( 2Meo bpy-Zn) by single-crystal X-ray diffraction, 2Meobpy represents 4,4′-dimethoxy-2,2′-bipyridine. The cytotoxicity results indicated that 2Meo bpy-Zn, unlike cisplatin, acts potently and selectively on the human breast cancer cells (MCF-7) compared to normal murine embryo cells (NIH/3T3) by IC50 value of 4.6 ± 0.5 µm and selectivity index (SI) of 2.0 over 48 h. 2Meo bpy-Zn and cisplatin showed anti-metastatic activity as evidenced by inhibition of the colony formation and cell migration. The flow cytometric assessment of MCF-7 cells supported that 2Meo bpy-Zn and cisplatin exert their cytotoxic effect through the apoptotic pathway. Moreover, 2Meo bpy-Zn could induce overproduction of intracellular reactive oxygen species (ROS) in MCF-7 cells. The apoptotic mechanism in 2Meo bpy-Zn-treated MCF-7 cells is probably related to the regulation of apoptosis-relevant genes expression, including BAX and BCL2. Moreover, 2Meo bpy-Zn is able to cleave pUC19 plasmid DNA through the hydrolytic reaction pathway. Finally, 2Meo bpy-Zn’s affinity towards antiapoptosis-related proteins, as a potential apoptosis inducer, as well as breast cancer-relevant proteins, as a potential anti-BC agent, was evaluated by in silico molecular docking studies. Altogether, the results of this work strongly evidenced that 2Meo bpy-Zn can be the subject of experimental validation and clinical trials to introduce this complex as a promising BC therapeutic agent. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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