Cholesterol crystals induce mechanical trauma, inflammation, and neo-vascularization in solid cancers as in atherosclerosis

Autor: George S. Abela, Venkat R. Katkoori, Dorothy R. Pathak, Harvey L. Bumpers, Monika Leja, Zain ul Abideen, Manel Boumegouas, Daniel Perry, Anas Al-Janadi, James E. Richard, Carlo Barnaba, Ilce G. Medina Meza
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: American Heart Journal Plus, Vol 35, Iss , Pp 100317- (2023)
Druh dokumentu: article
ISSN: 2666-6022
DOI: 10.1016/j.ahjo.2023.100317
Popis: Background and aims: Cancer and atherosclerosis share common risk factors and inflammatory pathways that promote their proliferation via vascular endothelial growth factor (VEGF). Because CCs cause mechanical injury and inflammation in atherosclerosis, we investigated their presence in solid cancers and their activation of IL-1β, VEGF, CD44, and Ubiquityl-Histone H2B (Ub-H2B), that promote cancer growth. Methods: Tumor specimens from eleven different types of human cancers and atherosclerotic plaques were assessed for CCs, free cholesterol content and IL1-β by microscopy, immunohistochemistry, and biochemical analysis. Breast and colon cancer cell lines were cultured with and without CCs to select for expression of VEGF, CD44, and Ub-H2B. Western blot and immunofluorescence were performed on cells to assess the effect of CCs on signaling pathways. Results: Cancers displayed higher CC content (+2.29 ± 0.74 vs +1.46 ± 0.84, p
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