Human Ribonuclease 6 Has a Protective Role during Experimental Urinary Tract Infection

Autor: Juan de Dios Ruiz-Rosado, Hanna Cortado, Macie Kercsmar, Birong Li, Gregory Ballash, Israel Cotzomi-Ortega, Yuriko I. Sanchez-Zamora, Sudipti Gupta, Christina Ching, Ester Boix, Ashley R. Jackson, John David Spencer, Brian Becknell
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Journal of Innate Immunity, Vol 15, Iss 1, Pp 865-875 (2023)
Druh dokumentu: article
ISSN: 1662-8128
00053473
23437731
DOI: 10.1159/000534736
Popis: Mounting evidence suggests that antimicrobial peptides and proteins (AMPs) belonging to the RNase A superfamily have a critical role in defending the bladder and kidney from bacterial infection. RNase 6 has been identified as a potent, leukocyte-derived AMP, but its impact on urinary tract infection (UTI) in vivo has not been demonstrated. To test the functional role of human RNase 6, we generated RNASE6 transgenic mice and studied their susceptibility to experimental UTI. In addition, we generated bone marrow-derived macrophages to study the impact of RNase 6 on antimicrobial activity within a cellular context. When subjected to experimental UTI, RNASE6 transgenic mice developed reduced uropathogenic Escherichia coli (UPEC) burden, mucosal injury, and inflammation compared to non-transgenic controls. Monocytes and macrophages were the predominant cellular sources of RNase 6 during UTI, and RNASE6 transgenic macrophages were more proficient at intracellular UPEC killing than non-transgenic controls. Altogether, our findings indicate a protective role for human RNase 6 during experimental UTI.
Databáze: Directory of Open Access Journals
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