| Autor: |
Zhu Hongfei, Chen Hongjun, Su Shuai, Wang Jingyan, Li Yanpeng, Sun Aijun, Ding Jiabo, Cui Zhizhong |
| Jazyk: |
angličtina |
| Rok vydání: |
2010 |
| Předmět: |
|
| Zdroj: |
Virology Journal, Vol 7, Iss 1, p 294 (2010) |
| Druh dokumentu: |
article |
| ISSN: |
1743-422X |
| DOI: |
10.1186/1743-422X-7-294 |
| Popis: |
Abstract Background The 1.8-kb mRNA was reported as one of the oncogenesis-related genes of Marek's disease virus (MDV). In this study, the bacterial artificial chromosome (BAC) clone of a MDV field strain GX0101 was used as the platform to generate mutant MDV to examine the functional roles of 1.8-kb mRNA. Results Based on the BAC clone of GX0101, the 1.8-kb mRNA deletion mutant GX0101Δ(A+C) was constructed. The present experiments indicated that GX0101Δ(A+C) retained a low level of oncogenicity, and it showed a decreased replication capacity in vitro and in vivo when compared with its parent virus, GX0101. Further studies in vitro demonstrated that deletion of 1.8-kb mRNA significantly decreased the transcriptional activity of the bi-directional promoter between 1.8-kb mRNA and pp38 genes of MDV. Conclusion These results suggested that the 1.8-kb mRNA did not directly influence the oncogenesis but related to the replication ability of MDV. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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