Inflammation and oxidative stress processes in induced precocious puberty in rats

Autor: Raluca Maria Pop, Luciana Mădălina Gherman, Elena-Mihaela Jianu, Ștefan Horia Roșian, Mădălin Mihai Onofrei, Lavinia Patricia Mocan, Veronica Sanda Chedea, Ioana Corina Bocsan, Dragoș Apostu, Andreea Roxana Todea, Eva Henrietta Dulf, Jeanine Cruceru, Carmen Mihaela Mihu, Alina Elena Pârvu, Anca Dana Buzoianu
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Heliyon, Vol 10, Iss 24, Pp e40962- (2024)
Druh dokumentu: article
ISSN: 2405-8440
69477442
DOI: 10.1016/j.heliyon.2024.e40962
Popis: This study aimed to assess the influence of different types of blue light sources on male and female rats' puberty onset, the morphologic-induced alterations in reproductive organs tissues, the impact on inflammation and oxidative stress markers, anxiety levels, and mathematical modeling for tissue data interpretation. Four groups of sixteen rats each (8 females and 8 males/group) were investigated: three groups were exposed to blue light from mobile phones (MP), computer screens (PC), or LED lamps (LED) versus the control group (CTRL). The rats in the CTRL group had no exposure while the other groups were exposed for 30 days to the blue light of MP, PC, and LED for 16 h per day. Serum levels of cortisol, TNF-α, IL-6, and MMP-2 and MMP-9 ovaries and testis tissue levels were analyzed using the ELISA technique. Total oxidative stress (TOS), nitric oxide (NO), and malondialdehyde (MDA) in serum were determined spectrophotometrically. Histomorphological examination was performed on both male and female genital organs. Rats of both sexes presented significant early onset of puberty secondary to blue light exposure. LED-emitted light significantly increased TNF-α and MMP-9 levels in both sexes. The MP and PC emitted light significantly affected the levels of MMP-2 in both females and males. Levels of TOS and NO were increased by LED, respectively by MP and LED exposure in female rats. The histopathological examination revealed no statistically significant differences in the ovaries and testes of rats across the different groups. Blue light exposure induces precocious puberty, by accelerating sexual maturation, and triggers the overproduction of MMPs that could promote organic alteration through tissue remodeling. Oxidative stress parameters were upregulated only in female rats, while cortisol levels were higher in male rats.
Databáze: Directory of Open Access Journals
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