The effects of foliar application of melatonin on some physiological and biochemical characteristics and expression of fatty acid desaturase gene in pistachio seedlings (Pistacia vera L.) under freezing stress

Autor: Adeleh Barand, Fatemeh Nasibi, Khosrow Manouchehri Kalantari, Mohammad Moradi
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Journal of Plant Interactions, Vol 15, Iss 1, Pp 257-265 (2020)
Druh dokumentu: article
ISSN: 1742-9145
1742-9153
17429145
DOI: 10.1080/17429145.2020.1781271
Popis: In this study, the impacts of exogenous melatonin on freezing stress mitigation in pistachio (Pistacia vera L.) seedlings (30-days old) were investigated. Our results showed that in pistachio seedlings under freezing stress antioxidant enzyme activity, malondialdehyde (MDA) hydrogen peroxide (H2O2), electrolyte leakage, chlorophyll degradation, proline and soluble sugar and γ-aminobutyric acid (GABA) content increased. Phenolic component, phenylalanine ammonia lyase (PAL) activity and the expression of fatty acid desaturase (FAD2) gene increased whilst ascorbate and glutathione concentration significantly decreased in comparison with control. In this research, application of melatonin significantly reduced the antioxidant enzyme activity, MDA, H2O2, chlorophyll degradation, GABA concentration, soluble sugar, and proline contents under stress condition. In addition electrolyte leakage, PAL activity and transcripts of FAD2 gene markedly decreased when the leaves of pistachio seedlings sprayed with melatonin. The results of this study showed that MEL could alleviate the oxidative damages induced by freezing stress and to maintain the structure and mobility of cell membrane in pistachio seedlings under stress conditions. Considering that MEL is an inexpensive and safe bio-stimulator, it seems that MEL is a potential candidate that could be applied used to increase the resistant pistachio trees against variety of biotic and abiotic stresses in the field conditions.
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