Serum mir-31-5p is a reliable biomarker in patients with oral squamous cell carcinoma and oral lichen planus

Autor: Nooshin Mohtasham, Zahra Ghorbani, Hossein Ayatollahi, Fatemeh Arab, Seyed Hamid Aghaee-Bakhtiari, Bashir Rasoulian, Farnaz Mohajertehran
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Egyptian Journal of Medical Human Genetics, Vol 25, Iss 1, Pp 1-6 (2024)
Druh dokumentu: article
ISSN: 2090-2441
DOI: 10.1186/s43042-024-00531-4
Popis: Abstract Background MicroRNAs have been proposed as a novel regulatory biomarker for gene expression and early diagnosis of cancers. In this study, we evaluate the expression level of miR-31-5p in the serum of patients with oral squamous cell carcinoma, oral lichen planus, and a healthy control group to obtain a primary diagnostic biomarker. Materials and methods Serum was collected from patients with oral lichen planus (n = 32), patients with oral squamous cell carcinoma (n = 35), and healthy subjects (n = 32). MicroRNA was isolated from serum and cDNA was made from it. Then, the quantitative and qualitative expression of miR-31-5p levels among the samples was checked by the qRT-PCR method. Results In this study, three groups were quantitatively and qualitatively analyzed for miR-31-5p expression in serum. The results showed that there was a statistically significant correlation between the mean quantitative and qualitative expression of miR-31-5p among the three groups (P
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