Promiscuous Behavior of HPV16E6 Specific T Cell Receptor Beta Chains Hampers Functional Expression in TCR Transgenic T Cells, Which Can Be Restored in Part by Genetic Modification
| Autor: | Kirsten B. J. Scholten, Janneke J. Ruizendaal, Marcus Graf, Thomas Schoedl, Duco Kramer, Chris J. L. M. Meijer, Stephen Man, Erik Hooijberg |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Cellular Oncology, Vol 32, Iss 1-2, Pp 43-56 (2010) |
| Druh dokumentu: | article |
| ISSN: | 1570-5870 1875-8606 |
| DOI: | 10.3233/CLO-2009-0493 |
| Popis: | Background: T cell receptor gene transfer is a promising strategy to treat patients suffering from HPV induced malignancies. Therefore we isolated the TCRαβ open reading frames of an HPV16E6 specific CTL clone and generated TCR transgenic T cells. In general low level expression of the transgenic TCR in recipient human T cells is observed as well as the formation of mixed TCRs dimers. Here we addressed both issues employing three different expression platforms. |
| Databáze: | Directory of Open Access Journals |
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