| Autor: |
ZHENG Xiaoyan, WANG Xingyun, ZHANG Yongjun |
| Jazyk: |
čínština |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Shanghai Jiaotong Daxue xuebao. Yixue ban, Vol 43, Iss 2, Pp 171-179 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1674-8115 |
| DOI: |
10.3969/j.issn.1674-8115.2023.02.005 |
| Popis: |
Objective·To study the effect of gasdermin D (GSDMD) inhibitor necrosulfonamide (NSA) on alveolarization arrest in lipopolysaccharide (LPS)-induced bronchopulmonary dysplasia (BPD) newborn rats via inhibiting alveolar epithelial cell pyroptosis.Methods·Pregnant SD rats were randomly assigned to four groups as follows: control, BPD, BPD with NSA and NSA group, and then were prepared to receive intra-amniotic injection of LPS. Lung tissues of newborn rats on the first, third and seventh day after birth were stained by hematoxylin-eosin (H-E) to observe lung development. The expressions of GSDMD-N-terminal in lungs of newborn rats in each group were detected by immunofluorescence. The mRNA levels of interleukin-1β (IL-1β) of newborn rats' lungs was detected by real-time PCR. In vitro, the mouse alveolar epithelial cell line MLE-12 was cultured and treated with LPS/adenosine triphosphate (ATP) and NSA. The cell viability of MLE-12 cells was detected by CCK-8 method, the pyroptosis was detected by Hoechst 33342 and propidium iodide (PI) staining, and the expressions of surfactant protein C (SFTPC) and GSDMD-N protein in MLE-12 cells were detected by immunofluorescence.Results·In vivo, intra-amniotic injection of LPS hindered lung development, resulting in the pathological hallmarks of BPD. The GSDMD-N expression of alveolar epithelial cells increased in the BPD rat model established by intra-amniotic injection of LPS, while NSA treatment significantly improved the lung development of BPD rats and inhibited the IL-1β mRNA expression (both P |
| Databáze: |
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| Externí odkaz: |
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