Genetic interaction mapping with microfluidic-based single cell sequencing.
| Autor: | John R Haliburton, Wenjun Shao, Adam Deutschbauer, Adam Arkin, Adam R Abate |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | PLoS ONE, Vol 12, Iss 2, p e0171302 (2017) |
| Druh dokumentu: | article |
| ISSN: | 1932-6203 02397943 |
| DOI: | 10.1371/journal.pone.0171302 |
| Popis: | Genetic interaction mapping is useful for understanding the molecular basis of cellular decision making, but elucidating interactions genome-wide is challenging due to the massive number of gene combinations that must be tested. Here, we demonstrate a simple approach to thoroughly map genetic interactions in bacteria using microfluidic-based single cell sequencing. Using single cell PCR in droplets, we link distinct genetic information into single DNA sequences that can be decoded by next generation sequencing. Our approach is scalable and theoretically enables the pooling of entire interaction libraries to interrogate multiple pairwise genetic interactions in a single culture. The speed, ease, and low-cost of our approach makes genetic interaction mapping viable for routine characterization, allowing the interaction network to be used as a universal read out for a variety of biology experiments, and for the elucidation of interaction networks in non-model organisms. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|