New perspective in the assessment of total intracellular magnesium

Autor:	Azzurra Sargenti, Lucia Merolle, Giulia Andreani, Concettina Cappadone, Giovanna Farruggia, Stefano Iotti, Chiara Marraccini
Jazyk:	angličtina
Rok vydání:	2014
Předmět:	total intracellular magnesium biological process DCHQ Biology (General) QH301-705.5
Zdroj:	Journal of Biological Research, Vol 87, Iss 1 (2014)
Druh dokumentu:	article
ISSN:	1826-8838 2284-0230
DOI:	10.4081/jbr.2014.2140
Popis:	Magnesium (Mg) is essential for biological processes, but its cellular homeostasis has not been thoroughly elucidated, mainly because of the inadequacy of the available techniques to map intracellular Mg distribution. Recently, particular interest has been raised by a new family of fluorescent probes, diaza-18-crown-hydroxyquinoline (DCHQ), that shows remarkably high affinity and specificity for Mg, thus permitting the detection of the total intracellular Mg. The data obtained by fluori- metric and cytofluorimetric assays performed with DCHQ5 are in good agreement with atomic absorption spectroscopy, confirming that DCHQ5 probe allows both qualitative and quantitative determination of total intracellular Mg.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/1a156f3ef4c349a6beb089c5080e27cc Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF