Prevalence, genotypes and risk factors for Toxoplasma gondii contamination in marine bivalve shellfish in offshore waters in eastern China

Autor: Wei Cong, Man-Yao Li, Yang Zou, Jun-Yang Ma, Bo Wang, Zhao-Yang Jiang, Hany M. Elsheikha
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Ecotoxicology and Environmental Safety, Vol 213, Iss , Pp 112048- (2021)
Druh dokumentu: article
ISSN: 0147-6513
DOI: 10.1016/j.ecoenv.2021.112048
Popis: We conducted a large-scale epidemiological investigation to detect the prevalence of Toxoplasma gondii in four marine bivalve shellfish species collected from six representative coastal regions of Weihai, eastern China. Between January 2018 and December 2018, 14,535 marine bivalve shellfish pooled into 2907 samples were randomly collected and examined for T. gondii DNA by a nested PCR assay targeting B1 gene. The results showed that 2.8% (82) of the 2907 pooled samples were tested positive for T. gondii DNA. Two T. gondii genotype (ToxoDB Genotype #9 and ToxoDB Genotype #1) were identified PCR-restriction fragment length polymorphism analysis. Factors that were found significantly associated with the presence of T. gondii DNA in marine bivalve shellfish included the source of samples (being wild) (odds ratio [OR], 3.34; 95% confidence interval [CI], 2.00–5.84; p
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