| Autor: |
Renfeng YU, Xiaocang ZOU, Dayang ZOU, Linhao LI, Kehui WANG, Xiaoming HE, Yaqing XU, Rihui QIN, Dongdong MO, Jiahui Duan, Tao YU, Wei LIU, Jinpeng GUO |
| Jazyk: |
čínština |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Zhongguo quanke yixue, Vol 25, Iss 14, Pp 1741-1749 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1007-9572 |
| DOI: |
10.12114/j.issn.1007-9572.2022.0166 |
| Popis: |
Background Based on the current prevalence of Coronavirus Disease 2019 (COVID-19) , early diagnosis, isolation, and treatment are important methods to prevent and control infectious diseases. The establishment of convenient and efficient immunochromatographic detection techniques is essential for the prevention and control of COVID-19 epidemic. Objective To establish a method for the detection of SARS-CoV-2 anti-N protein IgG antibody by immun of luorescence chromatography method based on quantum dots labeling technology in August, 2020. In order to determine whether the detected persons had been infected with COVID-19 or been injected with SARS-CoV-2 inactivated vaccine. Methods The prepared rat anti-human secondary antibody and anti-N protein antibody were immobilized on a Nitrocellulose (NC) membrane as detection line (T) and quality control line (C) , respectively. Then the SARS-CoV-2 N protein labeled by quantum dots was evenly sprayed on glass fiber, which was assembled, cut and packaged into test strips after drying. The test strips were used to detect the clinical serum of 35 COVID-19 patients and 50 healthy individuals, the results of the initial screening of the ELISA kit were used as a control to calculate the detection specificity and sensitivity of quantum dots fluorescence immunochromatography. The sensitivity of the test strip was detected by using the N protein antibody standard. Results The specificity and sensitivity of the strip were 100.00%, 94.29%, and the susceptibility was 8.53-17.06 ng/ml antibody concentration. Conclusion The detection of anti-N protein IgG antibody in serum by quantum dots labeling is simple, fast, with strong sensitivity and specificity. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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