Antibacterial activity of honeys and potential synergism of honeys with antibiotics and alkaloid extract of Sophora alopecuroides plant against antibiotic-resistant Escherichia coli mutant
| Autor: | Razieh Pourahmad Jaktaji, Farideh Ghalamfarsa |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Iranian Journal of Basic Medical Sciences, Vol 24, Iss 5, Pp 623-628 (2021) |
| Druh dokumentu: | article |
| ISSN: | 2008-3866 2008-3874 |
| DOI: | 10.22038/ijbms.2021.54224.12179 |
| Popis: | Objective(s): The increase in multidrug-resistant Escherichia coli strains with an overactivated AcrAB-TolC efflux pump has reduced the effectiveness of synthetic antibiotics, such as ciprofloxacin. The activity of this efflux pump can be reduced by using natural products. This study aimed to use a combination of ciprofloxacin, honey, and alkaloid extract of Sophora alopecuroides against an E. coli mutant with an overactivated AcrAB-TolC pump. Materials and Methods: First the physicochemical properties, total alkaloid content, antioxidant activity, and the minimum inhibitory concentration (MIC) of three local honey samples: Konar (lotus), Avishan (Thyme), and Gavan (Astragalus) were evaluated. Then, the MIC of different combinations of honey, ciprofloxacin, and plant alkaloid extract and expression of acrA and soxS genes were carried out using the agar dilution method and quantitative RT- PCR methods.Results: The net absorbance, total alkaloid content, and DPPH radical scavenging activity of Konar honey were significantly higher than those of Avishan and Gavan honeys (p |
| Databáze: | Directory of Open Access Journals |
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