Assessment of Adiponectin and Sperm Function Parameters in Obese and Non-Obese: A Comprehensive Study
| Autor: | Marziyeh Pooladi, Mohamadreza Sharifi, Gholamreza Dashti |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Cell Journal, Vol 24, Iss 12, Pp 715-722 (2022) |
| Druh dokumentu: | article |
| ISSN: | 2228-5806 2228-5814 |
| DOI: | 10.22074/cellj.2022.557337.1044 |
| Popis: | ObjectiveThe role of adiponectin in sperm function is inconclusive and there is a paucity of evidence. Obesity showsan ambiguous influence on sperm motility, and male subfertility. The aim of this study was to compare the role ofadiponectin and sperm functional parameters among obese and non-obese men.Materials and MethodsIn this comprehensive study, 64 male patients were included, and were classified asnon-obese [body mass index (BMI)< 24.9 kg/m2, n=32] and obese (BMI >25 kg/m2, n=32) groups. Sperm analysis,was conducted using World Health Organization (WHO) 2010 standards. Real-time polymerase chain reaction(PCR) and enzyme-linked immunosorbent assay (ELISA) were used for the analysis of adiponectin gene expressionand protein levels, respectively. Sperm viability was assessed using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT test), Acridine orange (AO) test was utilized to detect DNA denaturation, andsperm chromatin dispersion (SCD) technique was used to investigate the fragmentation of DNA.ResultsIn obese men, adiponectin gene expression (P |
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