| Autor: |
J M Ordovas, J P Peterson, P Santaniello, J S Cohn, P W Wilson, E J Schaefer |
| Jazyk: |
angličtina |
| Rok vydání: |
1987 |
| Předmět: |
|
| Zdroj: |
Journal of Lipid Research, Vol 28, Iss 10, Pp 1216-1224 (1987) |
| Druh dokumentu: |
article |
| ISSN: |
0022-2275 |
| DOI: |
10.1016/S0022-2275(20)38611-9 |
| Popis: |
A noncompetitive enzyme-linked immunosorbent assay (ELISA) has been developed for measuring total plasma apolipoprotein (apo) B using affinity purified polyclonal and monoclonal antibodies. Microtiter plates from different manufacturers were tested with regard to their IgG binding characteristics; only one plate yielded consistent coefficients of variation of less than 5%. The optimal plasma dilution in this assay was 1:3000. IgG anti-apoB antisera conjugated to alkaline phosphatase was used as a second antibody. p-Nitrophenyl phosphate was utilized as substrate for color development, and the absorbance (410 nm) was read utilizing an ELISA reader interfaced with a microcomputer for data processing. Plasma apoB levels in plasma have been determined in 1115 male and female participants in the Framingham Offspring Study. Mean (+/- SD) plasma concentrations were 89 +/- 28 mg/dl. Significant age and sex related differences in apoB levels were noted. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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