| Popis: |
Horned turban (Turbo cornutus) is an edible gastropod that occurs along the intertidal zone and basalt coastline, and is an important marine resource in Jeju, Korea. However, T. cornutus viscera are mostly discarded following processing. In this study, the antioxidant activity of viscera and muscle extracts was compared. In addition, the protective effect of T. cornutus viscera ethanol extract (TVEE) against H2O2-induced oxidative stress in human dermal fibroblasts (HDFs) was investigated. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and hydrogen peroxide scavenging activity of the viscera (IC50 = 0.64 ± 0.64 and 0.76 ± 0.01 mg/mL, respectively) showed higher activity than that in muscle. TVEE increased the reactive oxygen species (ROS) scavenging effect and cell viability in H2O2-induced HDFs without cytotoxicity. Furthermore, the fluorescence intensity of HDFs was high in those from H2O2-induced intracellular ROS production, but TVEE treatment decreased ROS production. H2O2 activated the mitogen-activated protein kinase (MAPK) signaling and matrix metalloproteinase 2 (MMP-2) in HDFs. However, MMP2 expression was confirmed to be reduced via MAPK (ERK, JNK, and p38) signaling phosphorylation. In conclusion, various antioxidant effects were confirmed in T. cornutus viscera, which instead of being discarded as marine by-products, can be applied as nutraceuticals in various industries. |
