Characterization of Differentially Expressed miRNAs by CXCL12/SDF-1 in Human Bone Marrow Stromal Cells
| Autor: | Potter Matthew L., Smith Kathryn, Vyavahare Sagar, Kumar Sandeep, Periyasamy-Thandavan Sudharsan, Hamrick Mark, Isales Carlos M., Hill William D., Fulzele Sadanand |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Biomolecular Concepts, Vol 12, Iss 1, Pp 132-143 (2021) |
| Druh dokumentu: | article |
| ISSN: | 1868-5021 1868-503X |
| DOI: | 10.1515/bmc-2021-0015 |
| Popis: | Stromal cell-derived factor 1 (SDF-1) is known to influence bone marrow stromal cell (BMSC) migration, osteogenic differentiation, and fracture healing. We hypothesize that SDF-1 mediates some of its effects on BMSCs through epigenetic regulation, specifically via microRNAs (miRNAs). MiRNAs are small non-coding RNAs that target specific mRNA and prevent their translation. We performed global miRNA analysis and determined several miRNAs were differentially expressed in response to SDF-1 treatment. Gene Expression Omnibus (GEO) dataset analysis showed that these miRNAs play an important role in osteogenic differentiation and fracture healing. KEGG and GO analysis indicated that SDF-1 dependent miRNAs changes affect multiple cellular pathways, including fatty acid biosynthesis, thyroid hormone signaling, and mucin-type O-glycan biosynthesis pathways. Furthermore, bioinformatics analysis showed several miRNAs target genes related to stem cell migration and differentiation. This study's findings indicated that SDF-1 induces some of its effects on BMSCs function through miRNA regulation. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|