| Autor: |
Ramin Mazhari, Shazia Ruybal-Pesántez, Fiona Angrisano, Nicholas Kiernan-Walker, Stephanie Hyslop, Rhea J. Longley, Caitlin Bourke, Catherine Chen, Deborah A. Williamson, Leanne J. Robinson, Ivo Mueller, Emily M. Eriksson |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Methods and Protocols, Vol 4, Iss 4, p 72 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2409-9279 |
| DOI: |
10.3390/mps4040072 |
| Popis: |
Serology tests are extremely useful for assessing whether a person has been infected with a pathogen. Since the onset of the COVID-19 pandemic, measurement of anti-SARS-CoV-2-specific antibodies has been considered an essential tool in identifying seropositive individuals and thereby understanding the extent of transmission in communities. The Luminex system is a bead-based technology that has the capacity to assess multiple antigens simultaneously using very low sample volumes and is ideal for high-throughput studies. We have adapted this technology to develop a COVID-19 multi-antigen serological assay. This protocol described here carefully outlines recommended steps to optimize and establish this method for COVID-19-specific antibody measurement in plasma and in saliva. However, the protocol can easily be customized and thus the assay is broadly applicable to measure antibodies to other pathogens. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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