| Autor: |
Muhammad Morshed, Min-Kuang Lee, Jonathan Laley, Darrel Cook, Annie Mak, Navdeep Chahil, Venessa Ryan, Carolyn Montgomery, Sylvia Makaroff, Sarah Malleson, Barbra Arnold, Troy Grennan, Jason Wong, Mel Krajden |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Microbiology Spectrum, Vol 10, Iss 3 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2165-0497 |
| DOI: |
10.1128/spectrum.00686-22 |
| Popis: |
ABSTRACT British Columbia (BC) implemented the syphilis reverse screening algorithm and Treponema pallidum PCR testing in 2014. We summarize the performance characteristics of the algorithm, together with PCR direct detection, and report on syphilis cases identified from 2015 to 2020. Prior to 2015, samples for syphilis diagnosis were first screened by rapid plasma reagin (RPR). As of 2015, sera were screened by the Siemens Advia Centaur syphilis assay (enzyme immunoassay [EIA]). Positive and equivocal samples were reflex tested by a T. pallidum passive particle agglutination assay (TPPA) and RPR. We used T. pallidum DNA PCR on clinical samples and restriction fragment length polymorphism analysis to identify azithromycin resistance mutations. Case/epidemiological data were obtained from the BC surveillance system. Of 1,631,519 samples screened by the EIA, 72,492 (4.4%) were positive and 187 ( |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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