C-SPAM: an open-source time-resolved specimen vitrification device with light-activated molecules

Autor:	Alejandra Montaño Romero, Calli Bonin, Edward C. Twomey
Jazyk:	angličtina
Rok vydání:	2024
Předmět:	c-spam tr-cryo-em sample preparation photochemistry-coupled devices millisecond resolution Crystallography QD901-999
Zdroj:	IUCrJ, Vol 11, Iss 1, Pp 16-22 (2024)
Druh dokumentu:	article
ISSN:	2052-2525 20522525
DOI:	10.1107/S2052252523010308
Popis:	Molecular structures can be determined in vitro and in situ with cryo-electron microscopy (cryo-EM). Specimen preparation is a major obstacle in cryo-EM. Typical sample preparation is orders of magnitude slower than biological processes. Time-resolved cryo-EM (TR-cryo-EM) can capture short-lived states. Here, Cryo-EM sample preparation with light-activated molecules (C-SPAM) is presented, an open-source, photochemistry-coupled device for TR-cryo-EM that enables millisecond resolution and tunable timescales across broad biological applications.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/18f955e6cb954adf8c07f6242db7a656 Zobrazit plný text záznamu View record in DOAJ