| Autor: |
LYU Xiaofan, WANG Guojian, ZHAO Na |
| Jazyk: |
čínština |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
陆军军医大学学报, Vol 46, Iss 11, Pp 1194-1205 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2097-0927 |
| DOI: |
10.16016/j.2097-0927.202401026 |
| Popis: |
Objective To detect the expression profile of heme oxygenase-1 (HO-1) during the process of wound repair in radiation-wound combined injury (R-W-CI), and evaluate its wound healing improving effects of R-W-CI by HO-1 knockdown with siRNA. Methods A total of 36 male C57BL/6J mice (8 weeks old) were randomly and equally divided into a simple skin wound group (W group) and a skin wound group combined with whole-body radiation (6 Gy) injury (R-W-CI group). During the wound healing process, the wounds were photographed and recorded, and the residual areas were quantified by Image J. Wound tissues were sampled and stained with HE staining for pathological and histological observation, and the damage to the hematopoietic system was assessed by dynamic examination of the peripheral blood. The expression and changes of HO-1 in wound tissues were detected by q-PCR and Western blotting. Then, 26 male C57BL/6J mice (8 weeks old) were randomly and equally divided into siRNA knockdown HO-1 group (si-HO-1 group) and siRNA negative control group (si-NC group). After radiation combined injury was inflicted, 60 μL of F127 gel loaded with si-HO-1 (5 μm/L) was applied to each wound in the si-HO-1 group, and an equal amount of F127 gel loaded with negative control si-NC was applied to the wound in the si-NC group. The knockdown of HO-1 in wound tissues was detected by Western blotting, and the changes in wound area were observed. In the wound tissues harvested in 3 d after wounding, the expression of cytokines IL-1β, IL-6 and TNF-α was examined by q-PCR and the proliferation of granulation tissues was evaluated by Ki67 immunohistochemical staining. HE staining was performed on wound tissues on day 3 and day 9 post-injury to assess the improvement effect of knockdown of HO-1 on wound healing of radiation combined injuries. Results Compared with the W group, semi-quantitative analysis of the residual wound area showed that healing was significantly delayed in the R-W-CI group on days 7 and 10 post-injury (P0.05), whereas statistical difference was seen in 10 d (P |
| Databáze: |
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| Externí odkaz: |
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