Metformin alleviates muscle wasting post-thermal injury by increasing Pax7-positive muscle progenitor cells

Autor: Yusef Yousuf, Andrea Datu, Ben Barnes, Saeid Amini-Nik, Marc G. Jeschke
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Stem Cell Research & Therapy, Vol 11, Iss 1, Pp 1-14 (2020)
Druh dokumentu: article
ISSN: 1757-6512
DOI: 10.1186/s13287-019-1480-x
Popis: Abstract Background Profound skeletal muscle wasting and weakness is common after severe burn and persists for years after injury contributing to morbidity and mortality of burn patients. Currently, no ideal treatment exists to inhibit muscle catabolism. Metformin is an anti-diabetic agent that manages hyperglycemia but has also been shown to have a beneficial effect on stem cells after injury. We hypothesize that metformin administration will increase protein synthesis in the skeletal muscle by increasing the proliferation of muscle progenitor cells, thus mitigating muscle atrophy post-burn injury. Methods To determine whether metformin can attenuate muscle catabolism following burn injury, we utilized a 30% total burn surface area (TBSA) full-thickness scald burn in mice and compared burn injuries with and without metformin treatment. We examined the gastrocnemius muscle at 7 and 14 days post-burn injury. Results At 7 days, burn injury significantly reduced myofiber cross-sectional area (CSA) compared to sham, p
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