Direct Interaction of Avian Cryptochrome 4 with a Cone Specific G-Protein

Autor: Katharina Görtemaker, Chad Yee, Rabea Bartölke, Heide Behrmann, Jan-Oliver Voß, Jessica Schmidt, Jingjing Xu, Vita Solovyeva, Bo Leberecht, Elmar Behrmann, Henrik Mouritsen, Karl-Wilhelm Koch
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Cells, Vol 11, Iss 13, p 2043 (2022)
Druh dokumentu: article
ISSN: 11132043
2073-4409
DOI: 10.3390/cells11132043
Popis: Background: Night-migratory birds sense the Earth’s magnetic field by an unknown molecular mechanism. Theoretical and experimental evidence support the hypothesis that the light-induced formation of a radical-pair in European robin cryptochrome 4a (ErCry4a) is the primary signaling step in the retina of the bird. In the present work, we investigated a possible route of cryptochrome signaling involving the α-subunit of the cone-secific heterotrimeric G protein from European robin. Methods: Protein–protein interaction studies include surface plasmon resonance, pulldown affinity binding and Förster resonance energy transfer. Results: Surface plasmon resonance studies showed direct interaction, revealing high to moderate affinity for binding of non-myristoylated and myristoylated G protein to ErCry4a, respectively. Pulldown affinity experiments confirmed this complex formation in solution. We validated these in vitro data by monitoring the interaction between ErCry4a and G protein in a transiently transfected neuroretinal cell line using Förster resonance energy transfer. Conclusions: Our results suggest that ErCry4a and the G protein also interact in living cells and might constitute the first biochemical signaling step in radical-pair-based magnetoreception.
Databáze: Directory of Open Access Journals
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