| Autor: |
Bonato Pierina S., Paias Fernanda O. |
| Jazyk: |
angličtina |
| Rok vydání: |
2004 |
| Předmět: |
|
| Zdroj: |
Journal of the Brazilian Chemical Society, Vol 15, Iss 2, Pp 318-323 (2004) |
| Druh dokumentu: |
article |
| ISSN: |
0103-5053 |
| Popis: |
We developed two sensitive and simple assay procedures based on high- performance liquid chromatography and capillary electrophoresis (CE) for the enantioselective analysis of omeprazole in pharmaceutical formulations. Rac-omeprazole and (S)-omeprazole were extracted from commercially available tablets using methanol:NaOH 2.5 mol L-1 (90:10, v/v). Chiral HPLC separation of omeprazole was obtained on a CHIRALPAK AD column using hexane:ethanol (40:60, v/v) as the mobile phase and detection at 302 nm. The resolution of omeprazole enantiomers by CE was carried out using 3% sulfated beta-cyclodextrin in 20 mmol L-1 phosphate buffer, pH 4.0 and detection at 202 nm. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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