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Blood M2a monocyte polarization and increased formyl peptide receptor 1 expression are associated with progression from latent tuberculosis infection to active pulmonary tuberculosis disease

Autor: Yung-Che Chen, Yu-Ping Chang, Chang-Chun Hsiao, Chao-Chien Wu, Yi-Hsi Wang, Tung-Ying Chao, Sum-Yee Leung, Wen-Feng Fang, Chiu-Ping Lee, Ting-Ya Wang, Po-Yuan Hsu, Meng-Chih Lin
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: International Journal of Infectious Diseases, Vol 101, Iss , Pp 210-219 (2020)
Druh dokumentu: article
ISSN: 1201-9712
DOI: 10.1016/j.ijid.2020.09.1056
Popis: Objectives: This study aims to explore the role of M2a polarization and formyl peptide receptor (FPR) regulation in the reactivation of Mycobacterium tuberculosis (Mtb) infection. Methods: M1/M2a monocyte percentage and FPR1/2/3 protein expression of blood immune cells were measured in 38 patients with sputum culture (+) active pulmonary TB disease, 18 subjects with latent TB infection (LTBI), and 28 noninfected healthy subjects (NIHS) using flow cytometry method. Results: M1 percentage was decreased in active TB versus either NIHS or LTBI group, while M2a percentage and M2a/M1 percentage ratio were increased. FPR1 expression on M1/M2a, FPR2 expression on M1, and FPR3 expression of M1 were all decreased in active TB versus LTBI group, while FPR1 over FPR2 expression ratio on NK T cell was increased in active TB versus either NIHS or LTBI group. In 11 patients with active TB disease, M1 percentage became normal again after anti-TB treatment. In vitro Mtb-specific antigen stimulation of monocytic THP-1 cells resulted in M2a polarization in association with increased FPR2 expression on M2a. Conclusions: Increased M2a and decreased M1 phenotypes of blood monocyte may serve as a marker for active TB disease, while decreased FPR1 on blood monocyte may indicate LTBI status.
Databáze: Directory of Open Access Journals
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