Ectopic Expression of AtYUC8 Driven by GL2 and TT12 Promoters Affects the Vegetative Growth of Arabidopsis

Autor: Chao Tan, Jia Song, Tan Zhang, Mengxiao Liang, Suxin Li, Huabin Liu, Shuzhen Men
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Seeds, Vol 2, Iss 3, Pp 278-289 (2023)
Druh dokumentu: article
ISSN: 2674-1024
DOI: 10.3390/seeds2030021
Popis: Auxin plays an essential role in regulating Arabidopsis growth and development. YUCCA (YUC) family genes encode flavin monooxygenases, which are rate-limiting enzymes in the auxin biosynthetic pathway. Previous studies showed that YUC8 overexpression (YUC8 OE), as well as ectopic expression of YUC8 driven by GL2 (GLABRA 2) and TT12 (TRANSPARENT TESTA 12) promoters, which are specifically expressed in the epidermis and inner seed integument, respectively, produced larger seeds compared to the Col. However, the impact of these transgenic lines on the vegetative growth of Arabidopsis remains unclear. Here, we show that the GL2pro:YUC8-GFP and TT12pro:YUC8-GFP transgenic plants produce a moderate excessive auxin accumulation phenotype compared to the YUC8 OE. These two transgenic lines produced smaller rosette and leaf, higher plant height, fewer branches, and longer siliques. These data will provide a basis for the study of the relationship between the ectopic expression of auxin synthesis genes and crop yield.
Databáze: Directory of Open Access Journals
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