A Molecular Imprinted Polymer as a Flow-Through Optical Sensor for Oxazepam

Autor: Roberta G. Machicote, Marcela A. Castillo, Maria E. Pacheco, Liliana Bruzzone
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Journal of Analytical Methods in Chemistry, Vol 2018 (2018)
Druh dokumentu: article
ISSN: 2090-8865
2090-8873
DOI: 10.1155/2018/6302609
Popis: A flow-through optosensing system for oxazepam recognition with fluorescence detection was performed by means of a molecular imprinted polymer based on its acid hydrolysis product, 2-amino-5-chlorobenzophenone. The synthesis was conducted via a noncovalent imprinting methodology, using methacrylic acid as a functional monomer and ethylene glycol dimethacrylate as a cross-linking agent. Hydrolysis (types and concentration of acids), polymer retention capacity, binding properties, and elution (selectivity and reversibility) conditions were optimized. The selected molecular imprinted polymer had a molar ratio composition of 1 : 6 : 45 (template : functional monomer : cross-linker). The proposed method was applied to the determination of oxazepam in a pharmaceutical formulation. External standard calibration, standard additions calibration, and Youden’s calibration were carried out in order to evaluate constant and proportional errors due to the matrix. The developed metabolite-based recognition system for benzodiazepines is an innovative procedure that could be followed in routine and quality control assays.
Databáze: Directory of Open Access Journals
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