Spatial distribution of live gut microbiota and bile acid metabolism in various parts of human large intestine
Autor: | Daisuke Chinda, Toshihiko Takada, Tatsuya Mikami, Kensuke Shimizu, Kosuke Oana, Tetsu Arai, Kazuki Akitaya, Hirotake Sakuraba, Miyuki Katto, Yusuke Nagara, Hiroshi Makino, Daichi Fujii, Kenji Oishi, Shinsaku Fukuda |
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Jazyk: | angličtina |
Rok vydání: | 2022 |
Předmět: | |
Zdroj: | Scientific Reports, Vol 12, Iss 1, Pp 1-18 (2022) |
Druh dokumentu: | article |
ISSN: | 2045-2322 18798888 |
DOI: | 10.1038/s41598-022-07594-6 |
Popis: | Abstract Gut microbiomics is based on analysis of both live and dead cells in the stool. However, to understand the ecology of gut microbiota and their symbiotic relationships with hosts, spatial distribution of live bacteria must be examined. Here, we analyzed the live composition of luminal microbiota (LM) and mucosa-associated microbiota (MAM) in the ascending and descending colons and the rectums of 10 healthy adults and compared it with the total composition. The abundance of Lachnospiraceae in live LM decreased along the gut length and was significantly lower than that in total LM. Contrastingly, the abundance of Bacteroidaceae and Bifidobacteriaceae in live LM was higher than that in total LM, suggesting differences in death rate during gut migration. Live Enterobacteriaceae levels in MAM were significantly higher in rectum than in the ascending and descending colons and in LM. High-performance liquid chromatographic analysis of luminal bile acids revealed that 7α-dehydroxylation occurred towards the rectum. In live LM where a bile acid-inducible gene could be detected, 7α-dehydroxylation rates were higher than those in the group without the gene. Overall, we showed differences in live bacteria composition among three gut sites and between LM and MAM, highlighting the importance of understanding their spatial distribution. |
Databáze: | Directory of Open Access Journals |
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