Conditional kisspeptin neuron-specific Kiss1 knockout with newly generated Kiss1-floxed and Kiss1-Cre mice replicates a hypogonadal phenotype of global Kiss1 knockout mice
| Autor: | Kana IKEGAMI, Teppei GOTO, Sho NAKAMURA, Youki WATANABE, Arisa SUGIMOTO, Sutisa MAJARUNE, Kei HORIHATA, Mayuko NAGAE, Junko TOMIKAWA, Takuya IMAMURA, Makoto SANBO, Masumi HIRABAYASHI, Naoko INOUE, Kei-ichiro MAEDA, Hiroko TSUKAMURA, Yoshihisa UENOYAMA |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | The Journal of Reproduction and Development, Vol 66, Iss 4, Pp 359-367 (2020) |
| Druh dokumentu: | article |
| ISSN: | 0916-8818 1348-4400 |
| DOI: | 10.1262/jrd.2020-026 |
| Popis: | The present study aimed to evaluate whether novel conditional kisspeptin neuron-specific Kiss1 knockout (KO) mice utilizing the Cre-loxP system could recapitulate the infertility of global Kiss1 KO models, thereby providing further evidence for the fundamental role of hypothalamic kisspeptin neurons in regulating mammalian reproduction. We generated Kiss1-floxed mice and hypothalamic kisspeptin neuron-specific Cre-expressing transgenic mice and then crossed these two lines. The conditional Kiss1 KO mice showed pubertal failure along with a suppression of gonadotropin secretion and ovarian atrophy. These results indicate that newly-created hypothalamic Kiss1 KO mice obtained by the Cre-loxP system recapitulated the infertility of global Kiss1 KO models, suggesting that hypothalamic kisspeptin, but not peripheral kisspeptin, is critical for reproduction. Importantly, these Kiss1-floxed mice are now available and will be a valuable tool for detailed analyses of roles of each population of kisspeptin neurons in the brain and peripheral kisspeptin-producing cells by the spatiotemporal-specific manipulation of Cre expression. |
| Databáze: | Directory of Open Access Journals |
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