| Popis: |
The review contains the generally accepted and most frequently used methods for studying soil nematodes communities in recent ecological studies. The methods of sampling, extraction, fixation, microslide mounting for morphological identification, methods of fixation of nematodes for the needs of isotopic analysis and molecular biological work are considered. The problems of metabarcoding of nematode communities are briefly discussed. Along with sample preparation methods, the ecological classification of nematodes and approaches to the analysis of data on the composition and abundance of their communities are considered. A description and principles for calculating the ecological indices of nematode communities are given, which allow us to get information about the degree of habitat disturbance, the state of the soil food web, and the predominant pathways of organic matter decomposition. Some problems and limitations of using such indices are considered. |
