Evaluation of antibacterial efficacy of fungal-derived silver nanoparticles against Enterococcus faecalis

Autor: Kiran Rahul Halkai, Jayashree A Mudda, Vasundhara Shivanna, Vandana Rathod, Rahul Halkai
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Contemporary Clinical Dentistry, Vol 9, Iss 1, Pp 45-48 (2018)
Druh dokumentu: article
ISSN: 0976-237X
0976-2361
DOI: 10.4103/ccd.ccd_703_17
Popis: Background: The main objective of endodontic therapy is complete elimination and prevention of bacteria from the root canal system; however, it is difficult due to anatomical ramifications of root canal system and growing resistant microbes to available disinfectants. Therefore, to overcome this problem, newer antimicrobial agents have to be developed. Aims: The aim of the study was to evaluate the antibacterial efficacy of fungal-derived biosynthesized silver nanoparticles (AgNPs) against Enterococcus faecalis. Materials and Methods: Freshly prepared silver nanoparticles using the endophytic fungi Fusarium semitectum, characterized by different techniques were used to evaluate the antibacterial efficacy against E. faecalis by agar well diffusion method measuring the zone of inhibition using different concentrations of nanoparticles (AgNPs) (A [20 μl], B [40 μl], C [60 μl], D [80 μl], and E [100 μl]), F (0.2% chlorhexidine [CHX]), G (2% CHX), H (ampicillin), and I (distilled water) were used as control groups. Statistical Analysis Used: One-way ANOVA followed by post hoc Tukey multiple comparison test was done. Results: AgNPs (100 ml) showed highest zone of inhibition 19.5 mm against E. faecalis. CHX (0.2%) 14.52 mm, CHX (2%) 20.02 mm, and ampicillin showed highest mean zone of inhibition 20.5 mm and distilled water showed no zone of inhibition. Results indicate no significant difference between E (100 μl), G (2% CHX), and H (ampicillin) (P < 0.0001). Conclusions: Biosynthesized AgNPs exhibit efficient antibacterial activity against E. faecalis and therefore can be used as root canal irrigant or intracanal medicament for root canal disinfection.
Databáze: Directory of Open Access Journals
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