| Autor: |
ZHANG Lijun, QI Feng, CHENG Zhiyong, ZHANG Zhao, WU Shijie, GUO Yantao, SUN Li'na, PENG Zhanxian, LIANG Wentong |
| Jazyk: |
čínština |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Zhongliu Fangzhi Yanjiu, Vol 46, Iss 1, Pp 63-67 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
1000-8578 |
| DOI: |
10.3971/j.issn.1000-8578.2019.18.0937 |
| Popis: |
Objective To explore the effect of interferon-alpha-2b(IFN-alpha 2b) on the expression of programmed death receptor-1(PD-1), programmed death ligand-1(PD-L1) and CD4+ CD25+ Foxp3+ regulatory T cell (Treg) in JAK2 V617F-positive myeloproliferative neoplasms(MPN) and related clinical significance. Methods We collected 61 cases of JAK2 V617F-positive MPN patients, including 41 cases as the newly diagnosed group, 20 cases as the IFN-α2b treatment group and 20 healthy volunteers as control group. JAK2 V617F/JAK2 ratio was detected by fluorescence quantitative polymerase chain reaction (FQ-PCR). The expression levels of PD-1 and PD-L1 in bone marrow and Treg in peripheral blood were detected by flow cytometry. The bone marrow and peripheral blood samples from 15 patients were selected and treated with 1×106U/L IFN-α2b for 48h; and then the expression levels of PD-1, PD-L1 and Treg were detected. Results The expression levels of JAK2 V617F, PD-1, PD-L1 and Treg in the newly diagnosed group were significantly higher than those in IFN-α2b treatment group and control group(P < 0.05). The expression levels of PD-1, PD-L1 and Treg in the patients with JAK2 V617F/JAK2 ratio≥50% were significantly higher than those with mutation rate < 50%(P < 0.05). JAK2 V617F burden was positively correlated with PD-1, PD-L1 in bone marrow and PD-1 in lymphocyte, while not correlated with Treg. The expression of PD-1, PD-L1 and Treg in primary MPN cells were inhibited by IFN-α2b after 48 hours(P < 0.05). Conclusion PD-1, PD-L1 and Treg participate in the pathogenesis of MPN together. Interferon could inhibit the progress of MPN via inhibiting the expression of JAK2 V617F, PD-1, PD-L1 and Treg. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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