| Autor: |
Thatyara Oliveira Monteiro, Antônio Gomes dos Santos Neto, Alan Silva de Menezes, Flávio Santos Damos, Rita de Cássia Silva Luz, Orlando Fatibello-Filho |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Biosensors, Vol 13, Iss 3, p 379 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2079-6374 |
| DOI: |
10.3390/bios13030379 |
| Popis: |
A sensitive and selective label-free photoelectrochemical (PEC) immunosensor was designed for the detection of cardiac troponin I (cTnI). The platform was based on a fluorine-doped tin oxide (FTO)-coated glass photoelectrode modified with bismuth vanadate (BiVO4) and sensitized by an electrodeposited bismuth sulfide (Bi2S3) film. The PEC response of the Bi2S3/BiVO4/FTO platform for the ascorbic acid (AA) donor molecule was approximately 1.6-fold higher than the response observed in the absence of Bi2S3. The cTnI antibodies (anti-cTnI) were immobilized on the Bi2S3/BiVO4/FTO platform surface to produce the anti-cTnI/Bi2S3/BiVO4/FTO immunosensor, which was incubated in cTnI solution to inhibit the AA photocurrent. The photocurrent obtained by the proposed immunosensor presented a linear relationship with the logarithm of the cTnI concentration, ranging from 1 pg mL−1 to 1000 ng mL−1. The immunosensor was successfully employed in artificial blood plasma samples for the detection of cTnI, with recovery values ranging from 98.0% to 98.5%. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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