Evaluating RHAU140-CFP molecule for specific recognition of parallel DNA G-Quadruplex by the fluorescence spectrophotometer
| Autor: | Trương Thị Tinh Tươm, Nguyễn Viết Chánh, Phan Thị Phượng Trang, Đặng Thanh Dũng |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ, Vol 18, Iss 2, Pp 5-12 (2023) |
| Druh dokumentu: | article |
| ISSN: | 2734-9322 2734-9594 |
| DOI: | 10.46223/HCMCOUJS.tech.vi.18.2.2661.2023 |
| Popis: | Determination of G-quadruplex formation in DNA is required for the design of small molecules (drugs) targeting G-quadruplex. In previous studies, we used the fluorescent protein RHAU53-CFP capable of recognizing parallel G-quadruplex structures through a fluorescent signal. Herein, we evaluated the ability to specifically recognize the parallel G-quadruplex DNA structure of RHAU140-CFP by fluorescence spectrophotometer. The results showed that the fluorescent protein probe was able to recognize the parallel G-quadruplex structure. In addition, the fluorescence signal showed that the sensitivity of the RHAU140-CFP protein was three times higher than that of the RHAU53-CFP fluorescent signal in determining the formation of a G-quadruplex structure. Fluorescence protein probe sensitivity would provide a potential tool for the detection of parallel G-quadruplex both in vitro and in vivo. |
| Databáze: | Directory of Open Access Journals |
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