The recovery and molecular identification of HAdV-D17 in raw sewage and mussel samples collected in the Eastern Cape province of South Africa

Autor:	Hillary J. Vos, Caroline M. Knox
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	human adenovirus (hadv) mussels nested pcr raw sewage Infectious and parasitic diseases RC109-216
Zdroj:	Southern African Journal of Infectious Diseases, Vol 33, Iss 1, Pp 4-7 (2018)
Druh dokumentu:	article
ISSN:	2312-0053 2313-1810
DOI:	10.4102/sajid.v33i1.27
Popis:	Human adenoviruses (HAdV) are a common cause of clinical infections in South Africa. However, there is a lack of information regarding the prevalence and molecular identification of this virus in the environment. The objective of this study was to investigate the recovery and molecular identification of HAdV in sewage and mussel samples. All samples were subjected to transmission electron microscopy, viral DNA extraction and nested PCR amplification using adenovirus-specific primers targeting a conserved region of the hexon gene. Amplicons were cloned and sequenced and BLAST analysis revealed a closest matched sequence (98% identity) belonging to HAdV-D17.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/17d7b9d321284a659dd0e817cc5ea077 Zobrazit plný text záznamu View record in DOAJ